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Contract Research Our working relationships encompass both fee for service arrangements as well as collaborative academic research. Our team of skilled individuals work in-house and with our clients, offering exceptional service capabilities which include designing a studies, conducting clinical trials and related value added experiments, data analysis and interpretation. We pay vital attention to time-lines and audit compliance |
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Triesta has taken on many challenging projects to work together with MNCs. Few study examples include:
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Evaluation of molecular markers for prognosis in Breast Cancer |
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Surveillance of common solid tumors for drug transporter proteins |
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Surveillance of common solid tumors for ABC transporter presence by gene expression and immunohistochemical detection: |
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Promoter methylation status of the MGMT gene in glioma patients as a predictor for response to Temozolamide |
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Validation of molecular markers for response to therapy in HCV patient. |
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Validation of molecular markers for response to drugs in NSCLC patients. |
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Evaluation of molecular markers in Pancreatic Carcinoma using archived Formalin Fixed Paraffin Embedded (FFPE) Tissue. |
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Analysis of Immunohistochemistry and Fluorescent images |
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Molecular markers predicting response to therapy in Breast Cancer |
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Her2-neu locus amplification and response to Trastuzumab.. |
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Storage of Biological samples (We offer a highly-controlled management solution for short-term and long-term temperature-controlled storage needs. Our outsourced services provide you with multiple, state-of-the-art, purpose-built biorepository, offering real-time tracking of stored biological samples and 24-hour retrieval of biomaterials. Secure storage is offered under the following conditions: |
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Ambient (20° C to 25° C) |
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Refrigerated (2° C to 8° C) |
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Frozen/Ultra-Low (-20° C to -70/-80° C) |
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Our sample storage services have: |
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Regulatory compliance |
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Guaranteed sample integrity |
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Secure inventory management through LIMS. |
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Same-day retrieval of materials |
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Cost savings |
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Tissue Micro Arrays. TMA are constructed by using a needle to biopsy a standard histologic sections and placing the core in an array on a recipient paraffin block. Hundreds or thousands of tissue cores are arranged on a single slide and then analyzed by a single immuno-staining experiment or in situ hybridization reaction. TMAs built are highly representative of donor tissues with confirmed pathological diagnosis. |
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Salient Features of TMAs over single sections: |
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TMA are constructed by using a biopsy needle from the donor blocks then placing the core in an array on a recipient paraffin block.
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TMAs conserve tissue resources and experimental reagents |
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Improve internal experimental controls |
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Increase sample numbers per experiment |
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Can be used for large-scale, massively parallel in situ analysis. |
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Triesta offers custom made TMAs with various tissue types and variable replicates depending on the requirement. |
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Biomarker Analysis.
Triesta's biomarker research is focused on the identification, measurement and clinical validation of novel DNA, RNA and protein-based targets and biomarkers at the level of gene expression and epigenetic changes.
Triesta offers its capabilities to partners and customers as a service or through research collaboration with shared risks and rewards:
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Biomarker discovery |
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Clinical validation / replication studies |
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Assay development and standardization |
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Extraction of DNA and RNA. Triesta offers variety of methods to extract top quality nucleic acids from various types of biological material not limited to FFPE blocks, fresh frozen tissue, blood, yeast, bacteria and cell culture. |
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Cell-culture: Triesta’s well established cell culture facilities are well equipped to maintain cell lines, primary cultures and transfection assays. |
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Mutational Analysis. We offer standardized methods of mutation detection either by direct DNA sequencing or ARMS PCR. Mutation of EGFR, K-ras and p53, to mention a few, are offered for diagnostic and research work. |
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FISH. EGFR and Her-2/neu gene amplification are being studied by Fluorescent in situ hybridization (FISH). |
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Cytogenetics. Conventional karyotyping, cell cultures, FISH on metaphase, solid tumor cytogenetics for diagnosis as well as for research. |
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Flow cytometry based assays. Triesta has the expertise for various flow cytometer based assays, not limited to immunophenotyping, DNA ploidy and drug resistance |
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LCM: The MMI SmartCut® system at Triesta is a tool to isolate clean and pure population of cells. This is achieved by dissecting the areas of interest with a UV-laser while maintaining morphology, ensuring quality of the source material and contamination free collections for subsequent downstream analysis. |
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Applications: |
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Differential analysis of genomic and proteomic expression of any tissue type. |
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Single cell isolation: Single cells can be easily identified and isolated from tissue sections, fine needle aspiration smears, blood and cell cultures |
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Live cells can be captured from a suspension and can be re-cultured without any adverse cellular effects. |
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